TABLE 5.

Effect of spo0J on chromosome content during sporulation^a

Strain	Region	soj spo0Jb	% of cells with indicated no. of focia						No. of sporangia analyzed
			0	1	2	3	4	>4
PSL62	359°	+	0.28	1.9	89	7.2	1.9	<0.28	360
PSL73		−	0.87	12	70	10	6.1	<0.29	346
PSL66	270°	+	0.55	1.7	94	2.8	1.4	<0.28	361
PSL76		−	1.6	6.5	62	17	12	1.6	551
PSL64	181°	+	0.71	32	63	1.7	0.9	<0.24	420
PSL74		−	1.0	21	65	6.0	5.7	0.7	402

^aThe indicated strains (all containing a null mutation in spoIIIE) were grown in defined minimal medium and induced to sporulate by the addition of mycophenolic acid. Samples were taken 4 h after the initiation of sporulation and stained with the membrane dye FM4-64 to allow identification of cells that had divided asymmetrically. The number of foci per sporangium (mother cell plus forespore) of LacI-GFP (or LacI-CFP) was determined for cells that had undergone an asymmetric septation. The percentage of sporangia with the indicated number of foci for each strain is presented. An array of lac operators was inserted in the indicated region of the chromosome and visualized with LacI-GFP or LacI-CFP.

^b+, wild-type soj and spo0J; −, soj spo0J double mutant.