Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 Mar;26(5):1654–1665. doi: 10.1128/MCB.26.5.1654-1665.2006

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2006, American Society for Microbiology

PMC Copyright notice

FIG. 1. — In vivo interaction of OGG1 and p300. A. Extracts of HCT116 cells cotransfected with expression plasmid for FLAG-tagged OGG1 and p300 were immunoprecipitated with p300 antibody (lane 2) or preimmune sera (lane 3) and then blotted with FLAG antibody. Lane 1, FLAG-tagged OGG1 in cell extract as marker. Lower panel, Western analysis with anti-FLAG antibody of cell extracts used in upper panel. B. Extracts of cells transfected with FLAG-tagged OGG1 were immunoprecipitated with FLAG antibody (lane 2) or preimmune sera (lane 3), and the immunoprecipitates were analyzed for p300 by Western blotting. Lane 1, cell extracts used as marker. C. Extracts of HCT116 cells were immunoprecipitated with p300 antibody (lane 2) or preimmune sera (lane 1) and then immunoblotted with OGG1 antibody; lanes 3 and 4, input controls of cell extracts. D. Colocalization of OGG1 and p300. MRC5 cells were immunostained with OGG1 (green) and p300 (red). E. Incision activity of p300 immunoprecipitates (lane 1) or preimmune sera (lane 2) with ³²P-labeled 8-oxoG·C oligonucleotide. Lower panel, Western analysis of immunoprecipitates with p300 antibody and input controls of cell extracts (lanes 3 and 4).