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. 2006 Mar;26(5):1654–1665. doi: 10.1128/MCB.26.5.1654-1665.2006

FIG. 2.

FIG. 2.

In vivo and in vitro acetylation of OGG1. A. Extracts of HCT116 cells transfected with FLAG-tagged OGG1 (lane 3) or empty vector-transfected cells (lane 2) and then labeled with [3H]sodium acetate were immunoprecipitated with FLAG antibody and analyzed by SDS-PAGE and fluorography. Lane 1, in vitro-acetylated 3H-labeled OGG1 marker. B. In vitro acetylation of full-length OGG1 with immunoprecipitated p300 (lane 2) or preimmune sera (lane 1) followed by SDS-PAGE and fluorography. Middle panel, Western analysis of immunoprecipitate with p300 antibody. Lower panel, Coomassie blue staining of the input OGG1 in a duplicate gel after SDS-PAGE. C. In vitro acetylation of full-length OGG1 (lane 1) or OGG1 with the C-terminal 20 amino acids deleted (CΔ20; lane 2) with purified p300 HAT domain and [3H]acetyl-CoA followed by SDS-PAGE and fluorography. * indicates autoacetylated p300 HAT. D. Coomassie blue staining of the duplicate gel after SDS-PAGE. M, molecular weight markers.