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. 2006 Mar;26(5):1654–1665. doi: 10.1128/MCB.26.5.1654-1665.2006

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FIG. 3. — In vivo acetylation of wild-type OGG1 and K338R/K341R mutant. A. Extracts of HCT cells transfected with FLAG-tagged WT OGG1 (lane 1) or FLAG-tagged K338R/K341R OGG1 (lane 2) or empty vector (lane 3) and then labeled with [³H]sodium acetate were immunoprecipitated with FLAG antibody and analyzed by SDS-PAGE and fluorography. Lane 4, in vitro-acetylated ³H-labeled OGG1 marker. B. Western analysis with FLAG antibody for the WT and K338R/K341R OGG1 in the immunoprecipitates and cell extracts used in panel A.