FIG. 6.

Noxa is involved in the promotion by AR on Bax-mediated apoptosis. (A) AR-negative PC3 cells were transfected with expression vector encoding AR or empty vector in the presence or absence of R1881 (1 nM). After 24 h, cells were harvested and subjected to immunoblotting analysis with antibodies against AR (C19), Bax, Bcl-2, Bid, Bak, Bad, Puma, Bim, and Noxa. (B) PC3 cells were transfected with expression vectors encoding WT AR or the AR(C619Y) mutant or empty vector for 24 h. Cell lysates were subjected to immunoblotting analysis with antibodies against AR and Noxa. (C and D) PC3 cells were cotransfected with AR, along with Noxa siRNA or control siRNA, for 24 h, followed by UV irradiation (10 mJ/cm²) for an additional 14 h. Cell lysates were subjected to immunoblotting analysis with antibodies against Noxa and AR (C); the apoptotic cell death was visualized and quantitated by nuclear staining with Hoechst stain (H33258) (D).