FIG. 1.

Deletion of the Ufo1 UIMs leads to G₁/S cell cycle arrest. (A) pGFP-UFO1 or pGFP-UFO1ΔUIM galactose-inducible transformants without (left side of plate) or with (right side of plate) ectopic expression of SKP1 were plated on glucose and galactose plates and photographed after 36 h growth. (B) Cells expressing pGFP-UFO1 show a normal morphology (i), whereas transformants expressing pGFP-UFO1ΔUIM arrest with long buds (ii) and resemble the skp1-3 G₁ mutant at the restrictive temperature (iii). (C) Terminal arrest phenotype of cells deleted for the UIMs of genomic UFO1.