FIG. 8.

The Ufo1UIMs interact with all three UbL-UbA proteins, whereas full-length Ufo1 interacts only with Ddi1. (A) The Ufo1 UIMs produced in yeast as a GFP fusion were incubated with immobilized ^GstDdi1, ^GstRad23, ^GstDsk2, or GST proteins purified from bacteria. TCL, total cell lysates. (B) Full-length ^GFPUfo1 was incubated with the same immobilized proteins. (C) An equal amount of ^GFPUfo1 yeast lysate was incubated with immobilized ^GstDdi1 in the presence of increasing amounts of yeast lysate with ^GFPUIMs.