FIG. 9.

Countering REST/NRSF function blocks cerebellar tumorigenicity. (A) Cell extracts prepared from Ad.GFP (G)- or Ad.REST-VP16 (RV)-infected NSC-M, NSC-M-V, and NSC-M-R cells were subjected to Western blot analysis in which antibodies were used that detect cleaved PARP (85 kDa) and α-tubulin (55 kDa). (B) NSC-M-R cells infected with Ad.GFP or Ad.REST-VP16 were inoculated into the mouse cerebellum. The mice were sacrificed 6 weeks later, and their paraffin-embedded brain sections were histologically analyzed. The tumor produced by NSC-M-R cells infected with Ad.GFP is indicated by arrows. (C) NSC-M and NSC-M-R cells infected with Ad.REST-VP16 were examined by immunofluorescence using anti-neuronal β-tubulin (red). The cell nuclei were also labeled with DAPI (blue). (D) NSC-M and NSC-M-R cells, uninfected (−) or infected (+) with Ad.REST-VP16, were examined by Western blotting analysis using anti-neuronal β-tubulin. Anti-α-tubulin was used as an internal control.