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Dependence of the silencing activity on the distance between the CTCF-binding site and the enhancer. The constructs are shown on the left; the 200-bp SmaI fragment under study is shown by the open box containing a filled circle showing the approximate position of the CTCF-binding site. The DNA fragments inserted between the βA/ɛ enhancer and the 200-bp fragment are shown by hatched boxes. Enhancer activity is presented as shown in Fig. 7.
