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. 2006 Mar;26(6):2175–2186. doi: 10.1128/MCB.26.6.2175-2186.2006

FIG. 2.

FIG. 2.

Level of myosin VI, but not GBF, GM130 and p115, is increased by DNA damage in a p53-dependent manner. (A) The induction of myosin VI by DNA damage is dependent on p53. RKO and RKO-p53-KD were untreated (−) or treated with dimethyl sulfoxide (DMSO), 0.2 μg/ml of doxorubicin (DOX), or 200 nM camptothecin (CPT). (B) The level of myosin VI protein is increased by DNA damage in a time-dependent manner. RKO cells were untreated (−) or treated with 0.2 μg/ml of DMSO, 0.2 μg/ml of doxorubicin, or 100 nM camptothecin for 12, 24, and 48 h. (C) The level of myosin VI, but not other Golgi proteins, is increased by DNA damage in a p53-dependent manner. RKO, LS174T, and HeLa cells were treated with 0, 0.1, 0.3, or 0.5 μg/ml of doxorubicin. Whole-cell extracts were analyzed by Western blotting with antibodies as indicated. Actin was analyzed as a loading control.