FIG. 3.
Increased proliferation and apoptosis caused by deletion of Mnt. (A) Total thymocytes from lck-Cre and lck-Cre-Mntflox/flox mice were gated for live cells, stained with monoclonal antibodies to CD4 and CD8, fixed, and stained with 4′,6′-diamidino-2-phenylindole (DAPI) for cell cycle analysis by FACS based on DNA content. Percent S phase is indicated on representative histograms (n = 3), and the table to the right shows mean values with standard deviations. (B) Increased apoptosis in mature thymocytes from lck-Cre-Mntflox/flox mice. Total thymocytes were stained for CD4, CD8, and annexin V, and representative histograms (n = 3) comparing annexin V-positive staining of the indicated sorted thymocyte subpopulations are shown. Numbers in each histogram represent the percentage of annexin V-positive populations in each group. The table shows mean values and standard deviations (*, P = 0.03; **, P = 0.04). CD4 apoptosis was not significantly different.