FIG. 4.

Thymocyte expression of proteins and genes regulated by Myc. (A) Western blot analysis of c-Myc, N-Myc, and several Myc effector proteins. Lysates from total thymocytes isolated from lck-Cre, lck-Cre-Mnt^flox/+, and lck-Cre-Mnt^flox/flox mice were immunoblotted with antibodies against the indicted proteins. (B) Quantitative RT-PCR analysis of Mnt and several Myc target genes in thymocytes from lck-Cre-Mnt^flox/flox mice and control lck-Cre mice. The n-fold change in gene expression in thymocytes of lck-Cre-Mnt^flox/flox mice was determined by comparing acidic ribosomal phosphoprotein (Arbp) PO-normalized gene expression levels in thymocytes of lck-Cre-Mnt^flox/flox and lck-Cre mice. RT-PCR was performed in triplicate on samples obtained from two different mice for each genotype. Standard deviations are shown.