FIG. 5.

Splenomegaly and lymphadenopathy in lck-Cre-Mnt^flox/flox mice. (A) Spleens from 12-month-old lck-Cre-Mnt^flox/flox and lck-Cre-Mnt^flox/+ littermates. (B) Hematoxylin-and-eosin-stained sections from the spleens shown in panel A. (C) Gross enlargement of sublumbar lymph node in a lck-Cre-Mnt^flox/flox mouse. (D) Widespread expression of the mitosis marker phosphorylated histone H3 in spleens of lck-Cre-Mnt^flox/flox mice. (E) Representative (n = 4) FACS profiles of CD4⁺ CD3⁺ and CD8⁺ CD3⁺ populations in total splenocytes from lck-Cre-Mnt^flox/flox and lck-Cre-Mnt^flox/+ littermates. Note the relative increase in the percentage of triple-negative cells (cells lacking CD4 and CD8 [lower left quadrant]) representing non-T-cell (NT) populations. Values indicate percentages of the different populations. (F) Absolute numbers of cells in different splenic subpopulations calculated from FACS data and estimated total splenocyte numbers (after red blood cell lysis) determined from four independent experiments. Values are means ± standard deviations (*, P = 0.02; **, P = 0.02).