FIG. 7.

Intestinal inflammation and liver damage in lck-Cre-Mnt^flox/flox mice. (A) Representative livers of lck-Cre-Mnt^flox/+ and lck-Cre-Mnt^flox/flox mice at 12 months of age. Note darkened regions consistent with necrosis. (B) Prussian blue staining of liver sections from lck-Cre-Mnt^flox/+ and lck-Cre-Mnt^flox/flox mice. Prussian blue stains iron-containing cells and indicates the presence of hemosiderin-containing macrophages (blue staining). The dark pink staining in lck-Cre-Mnt^flox/flox liver (arrow) shows the patchwork of infiltrating lymphocytes. (C) Hematoxylin-and-eosin-stained sections of the small intestine (jejunum) from lck-Cre-Mnt^flox/+ and lck-Cre-Mnt^flox/flox mice at 12 months of age. Note the granulomatous inflammation (arrow) in the intestine of a lck-Cre-Mnt^flox/flox mice. The asterisks show the location of crypts at the base of the villi. The lower panel shows ×40 magnification of granulomatous tissue featuring multinucleated giant cells (arrow). (D) Prussian blue staining of granulomatous tissue at ×4 and ×40 magnifications.