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. 2006 Mar;26(6):2019–2028. doi: 10.1128/MCB.26.6.2019-2028.2006

FIG. 2.

FIG. 2.

TSA represses HIF-1α independently of VHL and p53. A. VHL independence. RCC4-VHL+ and -VHL− cells were treated under normoxia and hypoxia conditions for 8 h in the presence or absence of TSA (300 nM), and cell lysates were analyzed by Western blotting. Tub, tubulin. B. Dose responses and time courses. Normoxic VHL− cells were exposed to increasing concentrations of TSA for 8 h (upper panels) or to 300 nM of TSA for different time periods (lower panels). C. Effect of SAHA on HIF-1α expression. VHL− cells were exposed to increasing concentrations of SAHA for 8 h. D. TSA suppresses gene expression in VHL− cells. VHL− cells were exposed to TSA for 8 h, and total RNA was analyzed by RT-PCR. E. p53 independence. HCT116 p53+ or p53− cells were exposed to TSA (300 nM) for 8 h, and cell lysates were analyzed for HIF-1α and p53 expression. VEGF, vascular endothelial growth factor.