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. 2006 Mar;26(6):2373–2386. doi: 10.1128/MCB.26.6.2373-2386.2006

FIG. 3.

FIG. 3.

RNA interference of TFRC1 expression abrogates cell proliferation and cell cycle progression. (A) Immunoblot analysis of TFRC1 levels 72 h after transfection with siRNA oligonucleotides in unsorted cell populations. α-Tubulin is used as a loading control. (B) Growth rates are diminished after suppression of TFRC1. Cell proliferation was measured in mock-electroporated cells or in cells electroporated with TFRC1 or control siRNAs. Error bars represent standard deviations from two independent measurements. At least three independently performed experiments yielded similar results. (C) FACS analysis of anti-TFRC1-FITC labeled cells 72 h after transfection with siRNA oligonucleotides. (D) Cell cycle profiles after knockdown of TFRC1. Cells were double labeled with 7-amino-actinomycin and an anti-TFRC1-FITC antibody. (E) Bar graphs representing mean values and standard deviations of each cell cycle phase derived from three independent experiments. (F) Cell size remains unaffected after RNAi-mediated suppression of TFRC1. Forward light scattering (FSC) histograms obtained by FACS analysis are shown.