TABLE 1.
Transcripts responsive to TFRC1 knockdowna
| Class | Gene product (gene name) | Fold change
|
|
|---|---|---|---|
| Microarray value | Real-time PCR validation | ||
| Cell cycle | Cell division cycle 20 (CDC20) | −3.29 | −3.17 |
| Cyclin B1 (CCNB1) | −3.24 | −2.53 | |
| Cyclin A2 (CCNA2) | −2.79 | −3.62 | |
| Cell division cycle (CDC2) | −2.86 | ||
| Growth arrest and DNA damage-inducible 45 alpha (GADD45A) | +2.66 | +2.90 | |
| Cyclin-dependent kinase inhibitor 1A (CDKN1A) | +2.56 | +1.51 | |
| Inhibitor of DNA binding 2 (ID2) | +2.24 | ||
| Apoptosis | BCL2 binding component 3 (BBC3/PUMA) | +3.05 | +2.18 |
| Annexin A4 (ANXA4) | +3.91 | +3.89 | |
| Annexin A1 (ANXA1) | +2.12 | ||
| Tumor necrosis factor receptor superfamily, member 6 (TNFRSF6) | +2.25 | ||
| p53 regulated | Cyclin-dependent kinase inhibitor 1A (CDKN1A) | +2.56 | +1.51 |
| p53 target zinc finger protein (WIG1) | +2.42 | +2.27 | |
| BCL2 binding component 3 (BBC3/PUMA) | +3.05 | +2.18 | |
| TP53-activated protein 1 (TP53AP1) | +2.43 | +2.13 | |
| Tumor protein p53-inducible nuclear protein 1 (TP53INP) | +3.72 | +4.68 | |
| DNA replication | DNA2 DNA replication helicase 2-like (DNA2L) | −2.49 | |
| Topoisomerase (DNA) II alpha (TOP2A) | −2.32 | −3.72 | |
| Polymerase (DNA directed), epsilon 2 (POLE2) | −2.29 | −2.79 | |
| Chromosome organization | BUB1 budding uninhibited by benzimidazoles 1 homolog (BUB1) | −3.05 | −3.16 |
| Centromere protein A (CENPA) | −2.51 | ||
| Centromere protein F (CENPF) | −2.45 | ||
a
Fold change represents expression difference between scrambled control siRNA-treated cells and TFRC1 (low)-depleted cells.