TABLE 1.
Wild type or mutant | Increased cytoplasmic localization with CRM1 | Increased nuclear localization with LMB | Increased nuclear localization at 48 h |
---|---|---|---|
GFP | − | − | − |
Bright | + | + | + |
395-601 mutant | ND | + | + |
238-601 mutant | ND | + | + |
500-562 mutant | − | + | + |
450-541 mutant | +/− | ND | ND |
K466A mutant | ND | − | − |
Y535F mutant | ND | + | + |
+, change of localization pattern in >20% of cells; +/−, change of localization pattern in >10% of cells; −, no change of localization pattern or change in <10% of cells; and ND, not determined.