TABLE 1.

Nuclear accumulation of Bright depends on reduced NES activity^a

Wild type or mutant	Increased cytoplasmic localization with CRM1	Increased nuclear localization with LMB	Increased nuclear localization at 48 h
GFP	−	−	−
Bright	+	+	+
395-601 mutant	ND	+	+
238-601 mutant	ND	+	+
500-562 mutant	−	+	+
450-541 mutant	+/−	ND	ND
K466A mutant	ND	−	−
Y535F mutant	ND	+	+

^a+, change of localization pattern in >20% of cells; +/−, change of localization pattern in >10% of cells; −, no change of localization pattern or change in <10% of cells; and ND, not determined.