FIG. 6.

PKCɛ translocation depends on PKCα activities. When cotransfected with DN PKCα-RFP, PKCɛ-GFP remained in the cytoplasm of all the TRH-stimulated cells analyzed (B). Under the same conditions, PKCβ1 and -δ translocation were not affected (A and C). (D) Summary of the total number of cells analyzed in each situation and the subcellular localizations of the different GFP fusion proteins. All the data presented in panels A, B, and C were obtained 48 h after the cotransfection by real-time recordings. In each experiment, it was checked (n = 5 cells for each PKC) that PKCβ1, PKCɛ, and PKCδ-GFP, transfected alone, were localized at cell-cell contacts for PKCɛ and at the entire membrane for PKCβ1 and -δ. Bar, 5 μm.