FIG. 3.

Transcriptional repression, nuclear localization, and homodimerization of PRISM. (A) PRISM is a transcriptional repressor. Results for cotransfections of a 5× GAL4-UAS luciferase reporter with the GAL4 DBD alone or with 50, 100, and 150 ng of GAL4-PRISM fusions are shown. All luciferase data are normalized to protein content following cell lysis. (B) Nuclear localization of PRISM. Immunocytochemistry of FLAG-PRISM-transfected COS-7 cells. FLAG-PRISM is directed to the nucleus by the carboxy-terminal Zn fingers. (C) PRISM forms homodimers. Immunoprecipitation (IP) experiments of epitope-tagged full-length PRISM and PRISM deletions in cotransfected COS-7 cells. Input controls are shown below the immunoprecipitation panels. IB, immunoblotting. The amino acid positions of the deletion constructs are indicated.