FIG. 5.

EWS-Fli1 activates gene expression from sites that support cooperative DNA binding with AP-1. (A) Schematic diagrams of the reporter genes. Two copies of the tandem Ets and AP-1 sites from the indicated genes were placed upstream of a minimal promoter to drive luciferase expression. As controls, either the two Ets sites (muEts [mutant Ets]) or the two AP-1 sites (muAP-1) in the reporter genes were mutated. WT, wild type. (B) The indicated reporters were then cotransfected into 3T3 cells along with either pBabepuro or pBabepuro-EWS-Fli1 and a Renilla luciferase control plasmid. Forty hours later, luciferase activity was determined. The results are the average of at least three independent experiments performed in triplicate. RLU, relative light units.