FIG. 7.

Inhibition of CDK12 alternative splicing activity by SR proteins. (A and B) Splicing assays of HEK293T cells transfected with 0.4 μg of pCEP4-E1a and 1.8 μg of the plasmids listed above the panels were performed. The splicing assays were then performed as described under Materials and Methods. The upper panel shows a representative autoradiograph of the results. The positions of the 13S, 12S, 10S, and 9S transcripts are indicated on the right. The mean values and standard deviations from three independent experiments are shown in the lower panel. * indicates P < 0.05 in comparison to the control (lane 1) and # indicates P < 0.05 between the pairs marked by connecting lines by paired t test.