FIG. 5.

Suppression of the cold sensitivity and HU sensitivity of the pol32Δ strain by pif1Δ. The following BY4741 isogenic strains were used: BY4741, WT; MB205, pol32Δ; MB201, pif1Δ; and MB206, pol32Δ pif1Δ. YPD plates were spotted with these strains and incubated at 30°C for 2 days (top, left panel) or at 16°C for 7 days (top right panel) as indicated. YPD plates were spotted with the same strains in the absence (lower left) or presence (lower right) of 38 mM HU and incubated at 23°C.