TABLE 2.
Frequency of GCRs
| Relevant genotypea | GCR rate (CanR 5-FOAR)b |
|---|---|
| Wild type | 3.5 × 10−10 (1) |
| pif1-m2 | 4.8 × 10−8 (137) |
| pif1-m2 dna2Δ | 2.5 × 10−8 (71) |
| dna2-1 | >3.5 × 10−10 (1) |
| dna2-2 | 7.0 × 10−9 (20) |
| dna2-K1080A | 1.8 × 10−9 (5) |
a
All strains were constructed for this study by standard genetic techniques and are isogenic with the wild-type strain 3615 MATa ura3-52 leu2Δ1 trp1Δ63 his3 Δ200 lys2 Bgl hom3-10 ade2-Δ1 ade8 hxt13::URA3:pif1-m2, strain 4344; pif1-m2 dna2Δ, strain 4344dna2Δ; dna2-1, strain 3615-2-1; dna2-2, strain 3615-2-2; and dna2-K1080A, strain 3615-K1080A. The dna2-K1080A mutant is defective in helicase motif I.
b
GCR rates were determined by fluctuation analysis of at least two experiments consisting of 5 or 11 cultures, and average values are reported. Numbers in parentheses indicate fold increase in GCRs compared to wild type.