TABLE 3.

Growth of various triple mutants in the absence of Dna2 and Pif1^a

Effect	dna2Δ pif1Δ genes
Lethal	hst3, rtf1, sae2, mre11, yen1, exo1, rnh35, rad27, rrm3, ctf4, sgs1, bre1
Sick	est2,b rpd3, rtt103, sap30, lge1, swd3, swd1, vid21
No effect	cdc73, caf20, hir3, htz1, paf1, rad18, rad51

^aAll mutations are synthetic lethal with dna2-1 and/or dna2-2. All mutations are deletions in the BY4741 background and were obtained by crossing MB203.3 with strains carrying the respective single mutations obtained from Invitrogen. Tetrad analysis was performed by standard techniques. At least 10 tetrads were dissected for each cross. Synthetic lethality was scored as the absence of viable triple mutant spores. Synthetic sickness was also scored and represents fewer than the expected number of triple mutants and slower growth than either the single mutant or the dna1Δ pif1Δ strain.

^bEnhanced senescence.