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Depsipeptide does not increase p53 phosphorylation at Ser15, Ser20, and Ser392 sites. A549 cells were treated with depsipeptide (0 to 0.1 μM) for 6 h, and then protein was extracted for Western blots by using anti-Ser15-p53 (A), anti-Ser20-p53 (B), and anti-Ser392-p53 (C). A549 cells were irradiated with gamma rays at 8 Gy in the left panel of each figure as p53 phosphorylation controls. α-Tubulin as a loading control is shown under every panel.
