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. 2006 Apr;26(7):2560–2569. doi: 10.1128/MCB.26.7.2560-2569.2006

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Copyright © 2006, American Society for Microbiology

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FIG. 2. — Cbx7-associated histone modifications. (A) Far Western blotting assay. The GST-Cbx7 CD binds to female ES cell (LF2) histone H3 through K9me3 and K27me3, demonstrated by peptide competitions (lanes 1 to 6); Cbx7 CD does not bind to recombinant H3 (lane 2); GST alone and caging aromatic mutants fail to associate (lanes 7 to 9); Ponceau for equal loading of total histones (bottom). (B) IPs of mononucleosomal extracts prepared from Cbx7-flag stable 293 cells versus control cells. Inputs in both cases show presence of all histone modifications; Cbx7-flag associated histones (IP) are marked predominantly by facultative heterochromatic modifications, including H3K9me2, H3K27me3, and H4K20me1. un, unmodified.