FIG. 5.
PML protein is necessary for inhibition of VLP-mediated expression by IFN-α. (A) Luciferase activity from cos7 cells that were treated with VLPs or transfected using CaPi carrying pDNA with ori cultured without or with IFN-α. (B) Luciferase activity from primary embryo fibroblasts isolated from wild-type (pml+/+) or pml−/− mice that were pretransfected with a control plasmid (pEGFP; open bars) or pCMVLT2 carrying the polyomavirus large T antigen (pyLT) gene and then treated with VLPs carrying pDNA with the polyomavirus ori cultured without (open and gray bars) or with IFN-α (closed bars). Data are representative examples of at least three independent experiments.