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Influence of AlexaFluor-488 pDNA labeling on VLP-delivered reporter gene activitya
| Treatment | Luciferase activity at 24 h
|
|
|---|---|---|
| Unlabeled pDNA | AlexaFluor-488-labeled pDNA | |
| Cy3-VLPs | 1,625,762 | 74 |
| CaPi | 159,278 | 17,930 |
Results are shown as luciferase activity at 24 h from cos7 cells treated with Cy3-VLPs or transfected using CaPi carrying either unlabeled or AlexaFluor-488-labeled pDNA with the ori (0.5 μg/5 × 104 cells).
