TABLE 3.

Number of VLPs delivered per cell nucleus in the absence or presence of viral replication factors or TSA^a

Parameter	Value for:
	3T3 cells			cos7 cells
	ori mutant without TSA (n = 121; silent)b	ori mutant with TSA (n = 123; active)	ori mutant without TSA (n = 78; silent)	ori mutant with TSA (n = 31; active)	ori† without TSA (n = 64; active)
No. of nuclei containing the following no. of VLPs
0	72	89	42	10	25
1	26	22	21	8	19
2	13	9	11	4	12
3	7	2	2	3	2
4	2	0	1	3	2
5	1	1	1	3	2
6	0	0	0	0	1
7	0	0	0	0	0
8	0	0	0	0	1
% of nuclei with VLPs	40.5	27.6	46.2	67.7	60.9
Avg. no. of VLPs/nucleusc	1.75	1.5	1.58	2.48	2.07

^aNumber of Cy3-fluorescent VLPs counted (as described in the legend for Fig. 2) in 3T3 or cos7 cells with VLPs carrying either ori-mutant or ori⁺ pDNA and incubated with or without TSA.

^bThe expression status was determined by luciferase assays of parallel cultures and is indicated as active or silent. n, total number of nuclei counted per cell line and treatment.

^cCalculated for VLP-positive nuclei only.