Table 2.

Metabolic, immunological, hemostatic, and proteolytic characteristics of 48-wk-old male mice

Analyte	E−/− × IL-10+/+	n	E−/− × IL-10−/−	n
Plasma proteins and lipids
Plasma protein (g/dL)	5.14 ± 0.12	12	4.79 ± 0.17	12
Total cholesterol (mM)	12.3 ± 0.5	7	7.9 ± 1.5a	9
Triglycerides (mg/dL)	1.4 ± 0.1	10	1.4 ± 0.1	10
Immune system status at baseline
Total IgG2a (AU)	0.810 ± 0.070	6	1.208 ± 0.046c	6
Total IgG1 (AU)	0.530 ± 0.023	6	0.556 ± 0.051	6
IL-12 (pg/mL)	65 ± 11	6	1826 ± 1654c	6
IFNγ (pg/mL)	223 ± 10	6	272 ± 12a	6
Immune response to antigenic challenge (HSA)
Anti-HSA IgG2a (AU)	3.71 ± 0.34	9	4.86 ± 0.19c	9
Anti-HSA IgG1(AU)	4.76 ± 0.25	9	3.28 ± 0.34b	9
CD4+ IFNγ+ (%)	5 ± 2	9	17 ± 4a	9
CD4+ IL-4+ (%)	10 ± 2	9	9 ± 3	9
Coagulation factors
Plasma prothrombin F1+2 (nM)	0.13 ± 0.01	8	0.30 ± 0.08b	10
Plasma tissue factor (mU/mg protein)	3.99 ± 1.40	8	2.75 ± 1.75	10
Aortic tissue factor (U/mg protein)	3.1 ± 0.4	8	6.0 ± 1.0a	10
Spleen MNC tissue factor (mU/mg protein)	2.78 ± 0.36	8	2.19 ± 0.22	10
Thrombosed coronary arteries (%)
Left ventricle portion (n° vessels)
Base (2.7 ± 0.3)	20.0 ± 8.1	10	70.0 ± 8.2b	10
Middle-high (6.8 ± 0.4)	13.3 ± 4.5	10	54.2 ± 6.3b	10
Middle-low (11.2 ± 0.6)	28.9 ± 3.1	10	54.2 ± 6.1b	10
Apex (7.8 ± 0.5)	5.3 ± 3.3	10	35.5 ± 5.9b	10
Proteolysis
MMP9 distribution (shoulder/total plaque)	0.53 ± 0.06	8	0.76 ± 0.07b	10
Aortic pro-MMP9 activity (AU/mg protein)	1708 ± 466	8	4374 ± 1141a	10
Spleen MNC pro-MMP9 activity (103 AU/107 cells)	141 ± 13	8	1097 ± 97a	10
Aortic pro-MMP2 activity (AU/mg protein)	7331 ± 516	8	7142 ± 442	10
Aortic MMP2 activity (AU/mg protein)	1937 ± 193	8	1688 ± 178	10

^aP < 0.05.

^bP < 0.01.

^cP < 0.001.

MNC, mononuclear cells.