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. Author manuscript; available in PMC: 2006 Apr 5.
Published in final edited form as: Cell. 2005 Sep 9;122(5):707–721. doi: 10.1016/j.cell.2005.06.029

Figure 5.

Figure 5

Utilization of IRF3 as a Coactivator of p65 Determines Gene-specific Sensitivity (A) Location of NF-κB sites in proximal promoter regions of Scyb9, Clic4, Nfkbia and Gro1, and their expression profiles in response to LPS in wild-type (WT) and IRF3−/− peritoneal macrophages. (B) Expression profiles of Scyb9, Clic4, Nfkbia and Gro1 in response to LPS and Dex in wild-type peritoneal macrophages. (C) Recruitment of IRF3 to the proximal promoter regions of Scyb9 and Clic4, but not Nfkbia or Gro1, in response to LPS. Recruitment of IRF3 was largely inhibited by Dex. Proximal regions of Scyb9, Clic4, Nfkbia and Gro1 promoters that includes an NF-κB site were analyzed by ChIP assays using the indicated antibodies. Crosslinking was performed 1 h after treatment with LPS and Dex. (D) Expression profiles of IP10, Clic4 and Nfkbia in response to LPS and Dex in control (WT) and MyD88−/− peritoneal macrophages. Gene expression was determined by real-time quantitative PCR.