Table 3.

In vitro ESAT-6 peptides pool response in terms of IFN-γ production evaluated simultaneously by ELISA and ELISPOT assays in HIV⁺ and HIV⁻ individuals with active TB

Patients Code	HIV Status	ELISAa (pg/mL per million PBMC)	ELISAa (score)	ELISPOTb (SFCs per million PBMC)	ELISPOTb (score)
1	+	70	−	55	+
2	+	215	+	265	+
3	+	63	−	100	+
4	+	0	−	30	−
5	−	215	+	145	+
6	−	750	+	60	+
7	−	163	+	215	+
8	−	1450	+	110	+
9	−	63	−	55	+
10	−	0	−	85	+
11	−	0	−	15	−
12	−	3275	+	155	+
13	−	0	−	10	−

^aFor the ELISA, the positive response cutoff was 76 pg/mL per million PBMC above negative controls (see Materials and Methods).

^bFor the ELISPOT assay, the positive response cutoff was 32 spot-forming cells per million PBMC above negative controls (see Materials and Methods). PBMC, peripheral blood mononuclear cells; SFC, spot-forming cells.