Table 4.
RT-PCR analysis of microdissected HRS cells for expression of rhoC, ATBF1, and p21SNFT
| Samples positive/samples analyzed
|
|||
|---|---|---|---|
| Samplesa | rhoC | ATBF1 | p21SNFT |
| HRS cells | |||
| Case 1 | 3/3 | 3/3 | 3/3 |
| Case 2 | 3/3 | 3/3 | 2/3 |
| Case 3 | 3/3 | 3/3 | 3/3 |
| Case 4 | 3/3 | 3/3 | 2/3 |
| Controls (cases 1 to 4) | |||
| KMH2 cells | 6/6 | 5/5 | 5/6 |
| Non-HRS cellsb | 3/19 | 1/12 | 0/12 |
| GC B cells | 0/6 | 0/6 | 0/6 |
| Buffer controls | 0/14 | 0/12 | 1/12 |
Groups of 50 HRS cells and 50 non-HRS cells (small cells isolated from the same tissue sections as the HRS cells) were analyzed. Pools of 50 GC B cells isolated from sections of 2 tonsils were analyzed as additional controls. Intact RNA in the tonsillar samples was confirmed by successful amplification of a fragment of the CD52 RNA from several GC B-cell samples (not shown). Buffer controls represent reaction tubes with buffer but without cells. Groups of 10 FACS-sorted KMH2 cells served as positive controls for cDNA synthesis and RT-PCR.
The few positive non-HRS cell samples may be due to cellular contamination of the samples by fragments from HRS cells or expression of the respective genes by some cells among the non-HRS cells.