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. 2003 May-Aug;9(5-8):154–165. doi: 10.2119/2003-00020.ono

Figure 7.

Figure 7

Analysis of the distal HMGA2 gene promoter region (flanking nt −871 to −1128 of the genomic sequence with respect to the translational start). A: Primer extension of the HMGA2 RNA prepared from WERI-Rb1 (lane 1) and Y79 (lane 2) cells using an oligonucleotide complementary to nt −853 to −879 in the distal promoter region of HMGA2 gene. RNA extracted from OSH50T cells (lane 3) was used as negative control template. Extension products were analyzed in a 7M urea/8% polyacrylamide gel. Sequencing ladder generated from the same primer was electrophoresed simultaneously as a marker. The Davidic star to the left of the sequence indicates the transcription start site at nt −979. B: Nucleotide sequence of the distal HMGA2 gene promoter region. Consensus transcription factor recognition sites are shown. The arrow depicts the location of primer annealed. The bent arrow indicates the mapped transcriptional start site. Restriction sites are underlined. Sequence of the CA repeat is shown in italic.