Table 6.
Peptide binding analysis with 9 most prevalent HLA-DR molecules with 5 peptides predicted by TEPITOPE not to bind promiscuouslya
| Binding capacity/IC50% (nM)
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Peptides | DR1 DRB1 *0101 | DR3 DRB1 *0301 | DR4w4 DRB1 *0401 | DR4w15 DRB1 *0405 | DR7 DRB1 *0701 | DR5w11 DRB1 *1101 | DR6w19 DRB1 *1302 | DR2w2 B1 DRB1 *1501 | DR2w2 B2 DRB5 *0101 | Bound molecules | Freqb |
| gp43(118-132) | 3952 | 4420 | 1885 | 4728 | 767 | 724 | 171 | 1432 | 1228 | 3 | 33% |
| gp43(161-175) | 68 | – | 8540 | 7163 | 9787 | 12477 | 165 | 405 | 1690 | 3 | 33% |
| gp43(244-258) | 4401 | – | – | – | 21540 | – | 16254 | 5170 | 22074 | 0 | 0% |
| gp43(259-271) | 20724 | 5044 | 16989 | 1854 | 7530 | 16257 | 5621 | 429 | 19765 | 1 | 11% |
| gp43(347-360) | – | – | – | 21038 | – | 12983 | 22876 | – | 7758 | 0 | 33% |
a
The results are expressed in IC50% (nM). Peptide binding assays performed as described in Table 2. Bold numbers = significant affinity threshold below 1000. Dash (–) means IC50% > 30000.
b
Freq = frequency.