Table 7.
Proliferation response of PBMC from 10 PCM patients to 5 gp43 peptides selected by TEPITOPE program not predicted to bind to any of the HLA-DR moleculesa
| gp43 (118-132)c |
gp43 (161-175)c |
gp43 (244-258)c |
gp43 (259-271)c |
gp43 (347-360)c |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Patientsb | 0.1 | 1.0 | 10.0 | 0.1 | 1.0 | 10.0 | 0.1 | 1.0 | 10.0 | 0.1 | 1.0 | 10.0 | 0.1 | 1.0 | 10.0 | gp43 10 μg | PHA |
| p2 | 5.4 | 4.5 | 2.8 | 2.0 | – | 2.0 | – | 2.0 | – | – | – | – | – | – | – | 43.5 | 111.4 |
| p3 | – | – | – | 2.4 | – | – | – | – | – | – | – | – | – | – | – | 57.6 | 288.6 |
| p4 | – | – | – | 10.5 | 10.2 | 7.1 | – | – | – | – | – | – | 3.0 | – | – | 51.3 | 294.8 |
| p10 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 14.3 | 216.6 |
| p11 | – | 2.4 | 2.0 | 6.9 | 6.8 | 6.9 | – | – | – | – | – | – | – | – | – | 26.7 | 130.9 |
| p12 | – | – | – | – | – | – | – | – | – | 3.0 | 5.3 | 4.8 | – | – | – | 28.4 | 421.1 |
| p20 | – | – | – | – | 2.4 | 2.8 | – | – | – | – | – | – | – | – | – | 95.5 | 521.7 |
| p21 | – | – | – | – | – | – | – | – | – | – | – | 2.0 | – | – | – | 346.1 | 409.3 |
| p22 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 84.9 | 452.7 |
| p23 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 5.2 | 114.1 |
a
Only positive stimulation index values (SI = 2) are shown. Dash (–) corresponds to SI < 2.
b
p = patient.
c
PBMC (105 cells/well) were stimulated by peptides at 0.1, 1.0, and 10.0 μM as described.