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. 2003 Jan;71(1):384–392. doi: 10.1128/IAI.71.1.384-392.2003

FIG. 4.

FIG. 4.

DNase I footprint of Ler protein on the upstream LEE5 regulatory region. Concentrations of Ler protein (in micrograms per milliliter) are indicated at the top. (A) DNase I protection of the LEE5 coding strand. The Ler protein protected a region of DNA from DNase I digestion from positions −190 to −73, which is represented by an open bar. A DNase I-hypersensitive site was observed at position −111 on the coding strand and is indicated by an arrow. (B) DNase I protection of the LEE5 noncoding strand. Ler protein protected a region of DNA from positions −190 to −79, which is represented by an open bar. At higher concentrations of Ler protein, the regions of protection extended to positions −60 and −221 on the coding and noncoding strands, respectively, as indicated by shaded bars.