FIG. 3.

Association of paxillin with the cytoskeleton and VLA-5 after ingestion of FN-treated staphylococci. (A to C) Paxillin in the cytoskeletal fractions from macrophages that ingested FN-treated or untreated Cowan I (A), that were treated with 200 μg of FN/ml (B), and that ingested FN-treated or untreated S. saprophyticus 10312 (C) were analyzed by Western blot analysis. (D) Immunoprecipitation of S. aureus- or S. saprophyticus-treated macrophage lysates by anti-VLA-5 antibody. S. aureus HLj with or without FN treatment or FN-treated S. saprophyticus was added to the macrophage culture for 2 min. Macrophage lysates were prepared and immunoprecipitated with anti-VLA-5 antibody as described in Materials and Methods. The immunoprecipitates were subjected to Western blot analysis with anti-paxillin antibody or anti-α₅ antibody.