Table 1.
Oligonucleotides and restriction enzymes used for SSCP analyses of the exonic and 593 bp promoter regions of the RXRB gene.
| Exon | Oligonucleotide sense/antisense | PCR product (bp) | Restriction enzyme; length of fragments (bp) |
| 01a | CGGTATCCCTACTCTCAGCCA/GCGGGATCCAGCCAGG | 164 | - |
| 01b | GGTGCGAAAAGAAATGCA/CACTGGCTCGCCTGCC | 202 | - |
| 02 | CTCTTGTGTGTCTGTGTGCCT/GGTTAGAGGATTGGAAGGTCA | 403 | AvaII 176/227 |
| 03 | GACTTCCCTATTTCCCCCAT/TCTCGGAGAAGAGGAGGCT | 207 | - |
| 04 | TGAAGGTGTCTCCATGCAAC/AGAAGGGCATGTGGTCTAAGA | 275 | BsmFI 126/149 |
| 05 | CTGATGAGGCCGTAAGGATA/GTGGATTGACCCCAACACT | 280 | Eco24I 130/150 |
| 06 | TCCATAACTCTTACCCCCGT/CTGGGTACGCAAGGTAAGG | 255 | MboII 102/153 |
| 07 | CCTGATTTCTGGCTCCTGAC/CCTCTATCTACATGCCAGCCT | 209 | - |
| 08 | GCTGAGCTGTGACCTTTGAGT/TCTCGGAGAAGAGGAGGCT | 199 | - |
| 09 | CTCCTCTCTTCCCTGCCAT/CCTCATAGCACTCCCCACC | 199 | - |
| 10 | CTCAATCCCCTTCTCCCAC/AGTGTGAGAAGCACCACGTC | 209 | - |
| Promoter (593 bp) | |||
| Sys1 | GGGCTTAATTCGACCCAA/GCATTTCTTTTCGCACC | 213 | - |
| Sys2 | GCTAACAGGCCGGAGGAGA/GGATTGATCGGAGGATTAGCT | 212 | - |
| Sys3 | CTCTCCTTTCCCGGTTTG/TCGTCTAGTTGGAAACCGAG | 234 | - |
| Sys4 | CTCTTTATCCCGAACCACCT/TCTCGCGGGATCTAAAGG | 221 | - |
| Sys5 | CATCACGCTGACCAGAGG/TGGTGAAAGATTAGTGTCCCA | 214 | - |