FIG. 4.
P. aeruginosa-induced production of CCL20 (a and b) by human mast cells is not inhibited by IL-10, but that of GM-CSF (c and d) is inhibited. Human CBMC (5 × 105 cells/ml) were stimulated with P. aeruginosa strain 8821 (mast cell-to-bacteria ratio, 50:1) in the presence or absence of various concentrations (5, 50, and 500 ng/ml) of IL-10 for 24 h. Cell-free supernatants were used to determine cytokine levels by ELISA. Mean results from triplicate assays are shown for each of two representative cell donors.