FIG. 5.
Differential effects of dexamethasone (Dex) on P. aeruginosa-induced CCL20 (a and b) and GM-CSF (c and d) production by human mast cells. Human CBMC (5 × 105 cells/ml) were treated with P. aeruginosa strain 8821 (mast cell-to-bacteria ratio, 50:1) in the absence (0 μM) or presence of various concentrations (0.1, 1, and 10 μM) of Dex for 24 h. Cell-free supernatants were used to determine cytokine levels by ELISA. Mean results from triplicate assays are shown for each of two representative cell donors.