FIG. 6.
Cyclosporine does not block P. aeruginosa-induced production of CCL20 (a and b) by human mast cells but blocks that of GM-CSF (c and d). Human CBMC (5 × 105 cells/ml) were treated with P. aeruginosa strain 8821 (mast cell-to-bacteria ratio, 50:1) in the absence (0 ng/ml) or presence of various concentrations (10, 100, and 1,000 ng/ml) of cyclosporine for 24 h. Cell-free supernatants were used to determine cytokine levels by ELISA. Mean ELISA results from triplicate well assays are shown for each of two representative donors.