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(A) R26NotchIC cells or parental control 46C cells were cultured in triplicate for 3 d in the presence of 4 μM PD184352, 5 μM SU5402, or in an equivalent concentration of DMSO diluent (“No inhibitor”) and the proportion of Sox1GFP+ cells analysed by FACS on day 3.
(B) Intact cultures were immunostained for Oct4 on day 5 to visualise undifferentiated ES cells.
