Table 5.
lin-35 and lin-15B enhance somatic transgene silencing by an RNAi dependent mechanism
RNAi* | GFP expression | % Complete expression | |
Complete† | Partial‡ | ||
control | 27 | 2 | 93% |
dcr-1; control | 15 | 0 | 100% |
rde-4; control | 19 | 0 | 100% |
lin-35; control | 1 | 11 | 8% |
lin-35; dcr-1 | 11 | 18 | 38% |
lin-35; rde-4 | 14 | 17 | 45% |
lin-15B; control | 2 | 14 | 13% |
lin-15B; dcr-1 | 30 | 2 | 94% |
lin-15B; rde-4 | 25 | 5 | 83% |
*RNAi experiments were performed as described in Materials and methods. †Number of worms with GFP expression in all hypodermal seam cells. ‡Number of worms with GFP expression only in their anterior and/or posterior seam cells, but not in the midbody seam cells.