TABLE 3.
Expression of iron acquisition genes in a LuxS-deficient backgrounda
Gene | Product(s) | Expression (fold) in JP2-12b |
---|---|---|
ftnAB | Ferritin, iron storage protein | >−50 |
afuA | Periplasmic ferric transport protein | −8 |
fecB | Putative ferric citrate transport protein | −10 |
tbp | Putative transferrin binding protein | −3 |
hbp | Putative hemoglobin binding protein | −2 |
hasR | Putative hemophore binding protein | −2 |
sidD | Putative enterobactin receptor | +10 |
frpB | Putative ferrichrome transporter | +3 |
Expression was determined by real-time PCR using primers specific for each of the indicated genes.
The levels of expression are indicated relative to those in strain JP2, which expresses a functional LuxS polypeptide (18).