TABLE 4.
Expression of afuA and ftnAB in an ArcB-deficient mutant of A. actinomycetemcomitans
| Gene | Cycle threshold (Ct) for straina:
|
||
|---|---|---|---|
| JP2 | JP2arcB1 | JP2arcB1YGS | |
| afuA | 17.4 | 21.8 (≈16-fold)b | 18.4 |
| ftnAB | 12.0 | 16.5 (≈24-fold) | 12.0 |
a
Cycle threshold (Ct) was determined from second derivative plots of real-time PCR data obtained using the data analysis software supplied with the Smart Cycler system.
b
Values in parentheses indicate the approximate reduction in transcript copy number of the indicated gene in the ArcB-deficient strain JP2arcB1 relative to the numbers in strains JP2 and JP2arcB1YGS.