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Effect of blebbistatin on force output and on the sensitivity of force to twitchin phosphorylation. Panel A shows the effect of addition of blebbistatin (10 μM) at pCa 5 as well as the subsequent phosphorylation of twitchin by addition of cAMP. Note that the addition of cAMP decreased force in pCa 5 almost to that present in pCa > 8 plus cAMP. Panel B shows a force trace from a similar experiment at pCa 6.
