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. 2006 Feb 10;90(9):3203–3207. doi: 10.1529/biophysj.105.078402

FIGURE 3.

FIGURE 3

(Left) CD spectra of 22-mer duplex DNA in the presence (dashed line) or absence (solid line) of the europium complex in Tris buffer (10 mM Tris, 100 mM NaCl, pH = 7.1). (A) polydAdTpolydAdT, (B) polydGdCpolydGdC, and (C) polydApolydT. DNA concentration was 60 μM in basepair; 1:2 ratio of [Eu]/[DNA] was used in the experiments. (Middle) DNA CD melting profiles measured in the presence (dashed line) or absence (solid line) of the europium complex at (D) 269 nm poly(dAT)2, (E) 252 nm polydGdCpolydGdC, and (F) 248 nm polydApolydT, respectively. (Right) Job plot of the europium complex with 22-mer DNA: (G) polydAdTpolydAdT, (H) polydGdCpolydGdC, and (I) polydApolydT. Total concentration of the complex (in units of Eu) and DNA (in units of basepair) was held constant at 60 μM over the course of the titration.